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作者在第Ⅰ报中曾用鼠胚肺单层细胞培养普氏立克次体获得成功,并描述了普氏立克次体在细胞中繁殖过程和形态学特点。但由于该细胞产量太少,不能满足实验室研究工作的需要。因此,我们又试用了九种组织单层细胞培养,从中选出了三种对普氏立克次体敏感的细胞,即鼠胚皮肌、鼠胚肺和 Detoit—6传代细胞。普氏立克次体虽能在鸡胚细胞中繁殖,但其形态学容易变成衰退小球形态,通过小白鼠感染试验,证明其毒力和致病性大大减低,往往不能使受染动物发病。采用鼠胚皮肌和鼠胚肺细胞培养时,则无上述缺点。而且鼠胚皮肌细胞来源容易,
In the first report, the authors used the mouse embryo lung monolayer cells to culture Plattsii rickettsia success, and described the Platts rickettsia reproduction in the cell process and morphological characteristics. However, due to the cell yield is too small, can not meet the needs of laboratory research. Therefore, we tested nine tissue monolayer cultures and selected three cells that are susceptible to Plattskar rickettsia: the murine embryo muscle, mouse embryo lung and Detoit-6 passaging cells. Plattsii rickettsia, although able to breed in chicken embryo cells, but its morphology easily become a recession ball morphology, through mouse infection test, proved its virulence and pathogenicity greatly reduced, often can not make infected animals Disease. The use of mouse embryo muscle and mouse embryo lung cell culture, then the above shortcomings. And mouse embryonic myocyte source easy,