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目的在分离、培养、鉴定人胎盘源间充质样干细胞(human placenta derived mesenchymal-like stem cells, HPMSCs)的基础上,进行了人胎盘源间充质样干细胞移植治疗大鼠纹状体出血的实验,探讨了其在治疗脑出血中可能的作用。方法利用自体未肝素化新鲜血回注法建立大鼠尾壳核出血模型;取SD雄性大鼠28只,随机分成5组:(1)假手术组(n=4);(2)自然恢复组(n=6);(3)对侧脑移植组(n=6);(4)同侧脑移植组(n=6);(5)同侧颈总动脉移植组(n=6)。将体外荧光标记的HPMSCs移植入大鼠纹状体出血模型,通过提高的躯体摆动实验(elevated body-swing test,EBST)和悬尾姿势实验(the postural tail-hang test)进行模型的神经功能评价,观察注射HPMSCs前后大鼠神经功能的变化;并通过病理切片检测及荧光显微镜观察了HPMSCs在大鼠脑内的存活以及迁移状况。结果HPMSCs跨越血脑屏障,并主要向脑出血组织周围定向迁移。EBST和Bederson评分结果表明,移植后第5d和第14d对侧脑移植组、同侧脑移植组和颈总动脉移植组神经功能修复明显优于自然恢复组。至移植后28d时为止,未发现肿瘤组织形成。结论人胎盘源间充质样干细胞能跨越血脑屏障并向病灶处定向迁移且未见成瘤现象,移植入脑出血模型结果表明其可促进动物的神经功能缺损的修复,具备临床应用的潜在价值。
Objective To isolate and culture human placenta derived mesenchymal stem cells (HPMSCs) based on human placenta derived mesenchymal stem cell transplantation for the treatment of striatal hemorrhage in rats , To explore its possible role in the treatment of cerebral hemorrhage. Methods 28 SD male rats were randomly divided into 5 groups: (1) sham operation group (n = 4); (2) spontaneous recovery (N = 6); (3) contralateral brain transplantation group (n = 6); (4) ipsilateral brain transplantation group (n = 6); . In vitro fluorescently labeled HPMSCs were transplanted into a rat striatal hemorrhage model and the neurological function of the model was evaluated by elevated body-swing test (EBST) and the postural tail-hang test The changes of neurological function were observed before and after injection of HPMSCs. The survival and migration of HPMSCs in rat brain were observed by pathological sections and fluorescence microscope. Results HPMSCs cross the blood-brain barrier and migrate mainly to the periphery of intracerebral hemorrhage. The results of EBST and Bederson scoring showed that the neurological function of the lateral brain transplantation group, the ipsilateral brain transplantation group and the common carotid artery transplantation group was significantly better than that of the natural recovery group on the 5th and 14th day after transplantation. Twenty-eight days after transplantation, no tumor formation was found. Conclusion Human placenta-derived mesenchymal stem cells can migrate across the blood-brain barrier and migrate to the lesion with no tumorigenesis. Transplantation into an intracerebral hemorrhage model indicates that it can promote the repair of neurological deficits in animals and has the potential of clinical application value.