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WRKY家族转录因子参与植物各种生物胁迫和非生物胁迫反应,同时也参与形态建成、物质代谢、种子休眠、衰老等过程。本研究在前期红掌疫病诱导转录组数据中筛选到一个WRKY家族基因,RT-PCR扩增到1 658 bp全长cDNA,包含一个1 035 bp的开放阅读框,生物信息学分析预测此基因是一个典型的WRKY转录因子,命名为Aan WRKY1(NCBI No.KY597634)。qRT-PCR证实该基因叶片中受疫病侵染上调表达,同时能够被SA、JA、ABA、ETH、GA等激素信号分子诱导,非处理条件下在花梗和茎中表达量最高。通过农杆菌介导洋葱表皮侵染法,瞬时表达AanWRKY1-eGFP,显示该基因编码蛋白定位于细胞核,进一步酵母单杂交实验,证明该基因具有离体转录激活活性。研究结果表明红掌AanWRKY1基因能够响应细菌性疫病及其他胁迫反应,具有应激转录调控的作用,该基因的鉴定为进一步解析其分子病理学作用机理提供依据。
WRKY family of transcription factors involved in various plant biotic stress and abiotic stress responses, but also involved in morphogenesis, material metabolism, seed dormancy, aging and other processes. In this study, a WRKY family of genes was screened from the pre-anthocyanidin-induced transcriptomic data. The full-length cDNA of 1 658 bp was amplified by RT-PCR and contained a 1 035 bp open reading frame. Bioinformatics analysis predicted that the gene was A typical WRKY transcription factor, named Aan WRKY1 (NCBI No. KY597634). qRT-PCR confirmed that the gene was up-regulated in the infected leaves and was induced by hormone signaling molecules such as SA, JA, ABA, ETH and GA. The highest expression was found in pedicel and stem under untreated conditions. Agrobacterium tumefaciens-mediated onion epidermal infection method, transient expression of AanWRKY1-eGFP, the gene encoding protein located in the nucleus, further yeast single hybridization experiments, that the gene has ex vivo transcriptional activation activity. The results showed that the anthurium AanWRKY1 gene can respond to bacterial diseases and other stress responses and play a role in stress transcriptional regulation. The identification of this gene provides the basis for further analysis of its molecular pathological mechanism.