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目的 :观察蝎毒抗癌多肽 (APBMV)对肝肿瘤的抑制作用。方法 :噻唑蓝 (MTT)法、台盼蓝拒染法、集落形成以及H2 2 荷瘤小鼠模型。结果 :APBMV导致SMMC - 772 1细胞代谢MTT的能力显著低于对照组 ,APBMV的IC50 为 11 33μg/mL ;APBMV能显著抑制该细胞的生长 ,剂量 -效应关系明显 ,其IC50 分别为 15 87μg/mL、13 0 5 μg/mL和 8 70 μg/mL ;当APBMV的浓度 >8μg/mL时 ,SMMC - 772 1细胞的集落形成率显著低于对照组。APBMV能明显抑制H2 2 带瘤小鼠肿瘤的生长 ,生长抑制率达 37 31% (P <0 0 1) ,H2 2 带瘤小鼠外周血白细胞计数和脾脏指数无明显变化或略高于对照组 ,而 5 -Fu治疗组H2 2 带瘤小鼠白细胞计数和脾脏指数均明显下降(P <0 0 1)。结论 :APBMV是存在于东亚钳蝎蝎毒中的一种有效低毒的抗肿瘤成分 ,能够抑制SMMC - 772 1和H2 2 两种肝癌细胞的生长。
Objective: To observe the inhibitory effect of scorpion venom anti-cancer polypeptide (APBMV) on liver tumors. Methods: MTT method, trypan blue exclusion method, colony formation, and H2 2 tumor-bearing mouse model. RESULTS: APBMV resulted in the ability of SMMC-7721 cells to metabolize MTT significantly lower than that of the control group. The IC50 of APBMV was 11 33 μg/mL; APBMV significantly inhibited the growth of the cells, and the dose-response relationship was significant. The IC50 was 15 87 μg/mL, respectively. mL, 135 μg/mL and 870 μg/mL; when the APBMV concentration was >8 μg/mL, the colony formation rate of SMMC-7721 cells was significantly lower than that of the control group. APBMV significantly inhibited the growth of H2 2 tumor-bearing mice with a growth inhibition rate of 37 31% (P < 0 01). H2 2 tumor-bearing mice had no significant changes in peripheral white blood cell count and spleen index or were slightly higher than controls. In the group, the white blood cell count and spleen index of the H2 2 tumor-bearing mice in the 5-Fu-treated group were significantly decreased (P < 0 01). CONCLUSION: APBMV is an effective and low-toxicity anti-tumor component found in Toadstool poisoning. It inhibits the growth of SMMC-7721 and H2 2 hepatoma cells.