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作者建立了对DNA和RNA抗体非常敏感,特异和可重复的固相放射免疫试验,以此测定了SLE病人和正常人外周血单个核细胞(PBMC)体外培养上清中自然产生的抗DNA和RNA抗体。被研究的SLE病人25例、正常人对照者17名。结果发现,SLE病人的PBMC在体外比正常人的PBMC产生的IgG类抗DNA和抗RNA抗体多(P<0.01),但两者所产生的IgM类抗DNA和抗RNA抗体的量类似。然而,IgM类抗DNA抗体是抗变性DNA上决定簇的抗体,只有SLE病人的PBMC(53%)产生抗天然
The authors established a solid-phase radioimmunoassay that is sensitive, specific and repeatable for DNA and RNA antibodies to determine the naturally occurring anti-DNA and / or anti-tumor activity of in vitro culture supernatants of peripheral blood mononuclear cells (PBMC) from patients with SLE and normal individuals RNA antibody. Twenty patients were studied SLE, 17 normal controls. The results showed that PBMC from SLE patients produced more IgG anti-DNA and anti-RNA antibodies (P <0.01) in vitro than normal human PBMCs, but both produced similar amounts of IgM anti-DNA and anti-RNA antibodies. However, IgM anti-DNA antibodies are determinants of anti-degenerative DNA, and only PBMCs (53%) from patients with SLE develop anti-natural