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目的探讨胃黏膜定植乳酸杆菌对幽门螺杆菌感染及胃黏膜菌群数量的影响。方法收集130例慢性胃炎患者胃窦黏膜组织,病理学检测幽门螺杆菌,提取胃黏膜基因组DNA,采用荧光定量PCR法检测乳酸杆菌和总细菌数。结果幽门螺杆菌阳性组和阴性组的乳酸杆菌检出率和乳酸杆菌数(Log)差异均无统计学意义(P>0.05);乳酸杆菌阳性者和阴性者之间胃黏膜总细菌数(Log)差异无统计学意义(P>0.05);乳酸杆菌细菌数(Log)与胃黏膜总细菌数(Log)无显著相关性(P>0.05);不同炎症程度胃炎患者乳酸杆菌检出率差异无统计学意义(P>0.05);肠化组和未肠化组乳酸杆菌检出率差异无统计学意义(P>0.05);但是重度胃炎组幽门螺杆菌感染率和总细菌数量均显著高于轻度(P<0.05)和中度胃炎组(P<0.05)。结论胃黏膜定植乳酸杆菌对幽门螺杆菌的感染无影响,其存在与否及细菌数量对胃黏膜总细菌数亦无明显影响,并与胃炎炎症程度及肠化无关。乳酸杆菌不能通过抑制幽门螺杆菌定植、调节胃黏膜菌群而减轻炎症反应。
Objective To investigate the effect of gastric mucosal Lactobacillus on Helicobacter pylori infection and gastric mucosal flora. Methods Gastric mucosa were collected from 130 patients with chronic gastritis. Helicobacter pylori was detected by pathology. Gastric mucosa genomic DNA was extracted. Lactobacillus and total bacteria were detected by fluorescence quantitative PCR. Results There was no significant difference in the detection rates of Lactobacillus and Lactobacillus (P <0.05) between H. pylori-positive and -negative groups and between H. lactobacillus positive and negative ) Had no significant difference (P> 0.05). There was no significant correlation between the number of bacteria in Lactobacillus and the total number of bacteria in gastric mucosa (P> 0.05). There was no difference in the detection rate of lactobacilli among patients with different degree of inflammation (P> 0.05). There was no significant difference in the detection rate of lactobacilli between the intestinal metaplasia group and the non-intestinal metaplasia group (P> 0.05), but the H. pylori infection rate and the total bacterial count in severe gastritis group were significantly higher than Mild (P <0.05) and moderate gastritis (P <0.05). Conclusions Gastric mucosa-implanted Lactobacillus has no effect on the infection of Helicobacter pylori, its presence or absence and the number of bacteria have no significant effect on the total number of gastric mucosa, and has nothing to do with the degree of gastritis inflammation and intestinal metaplasia. Lactobacillus can not inhibit the helicobacter pylori colonization, regulating gastric mucosal flora and reduce inflammation.