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用斑点杂交试验直接检测202例不同类型肝炎及携带者的血清H-BV-DNA,总阳性率为51.47％,除肝炎后肝硬化外,各型肝炎及携带者间均无差异。在202例中,HBsAg阳性185例,HBsAg阴性17例,其HBV-DNA阳性率分别为55.1％和12.6％。在HBsAg、HBeAg和抗-HBC阳性的89例中,HBV-DNA阳性率为93.26％;HBsAg、抗-HBe、抗-HBc阳性34例及抗-HBe、抗-HB-c阳性17例中,HBV-DNA阳性率为23.53％;HBsAg、抗-HBe阳性、e系统阴性62例中,HBV-DNA阳性率11.29％。20例正常人血清阴性。6例携带者在抗病毒药物治疗中,全部HBV-DNA转阴,其中5例HBeAg继HBV-DNA转阴后阴转。检测结果表明本法高度特异、敏感,是判断乙肝病毒复制及血清传染性的可靠指标。
The total positive rate of serum H-BV-DNA in 202 cases of different types of hepatitis and carriers was 51.47% by dot blot hybridization test. There was no difference between hepatitis B carriers and carriers except hepatitis cirrhosis. Among 202 cases, 185 cases were positive for HBsAg and 17 cases were negative for HBsAg. The positive rates of HBV-DNA were 55.1% and 12.6% respectively. The positive rate of HBV-DNA was 93.26% in 89 cases of HBsAg, HBeAg and anti-HBC positive, 34 cases of HBsAg, anti-HBe, anti-HBc and 17 cases of anti-HBe and anti- HBV-DNA positive rate was 23.53%; HBsAg, anti-HBe positive, e-negative 62 cases, the positive rate of HBV-DNA was 11.29%. 20 normal serum negative. In 6 cases of carriers in the treatment of antiviral drugs, all HBV-DNA negative, of which 5 cases of HBeAg after negative conversion of HBV-DNA negative. The test results show that this method is highly specific and sensitive, is a reliable indicator of hepatitis B virus replication and serum infectivity.