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目的:研究通心络超微粉以及7-硝基吲唑(7-Nitroindazole,7-NI)对脑缺血再灌注大鼠海马一氧化氮(NO)产生的影响。方法:大鼠前脑缺血采用四血管阻断法,实验分为生理盐水组、通心络超微粉组以及7-NI组。选择性NO测定电极测定NO的浓度。结果:通心络超微粉以及7-NI没有影响大鼠的血压和海马的流量,与对照组相比,均显著减少缺血再灌注时海马内NO的产生(均<0.001)。结论:通心络超微粉、7-NI可以通过抑制神经型一氧化氮合酶(nNOS)从而减少NO的产生而起到神经保护的作用。
Objective: To study the effects of Tongxinluo superfine powder and 7-Nitroindazole (7-NI) on nitric oxide (NO) production in the hippocampus of rats with cerebral ischemia / reperfusion. Methods: The forebrain ischemia was induced by four-vessel occlusion in rats. The experiment was divided into normal saline group, Tongxinluo superfine powder group and 7-NI group. The NO selective electrode measures the concentration of NO. Results: Tongxinluo superfine powder and 7-NI had no effect on blood pressure and hippocampal flow in rats. Compared with the control group, both NO and 7-NI significantly reduced NO production in hippocampus during ischemia-reperfusion (all <0.001). Conclusion: Tongxinluo superfine powder, 7-NI can play a neuroprotective role by inhibiting the production of nitric oxide synthase (nNOS) and thereby reducing NO production.