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为探讨儿童肺炎支原体 (MP)感染的早期诊断 ,用荧光定量PCR(FQ -PCR)技术检测1010例疑似MP感染患儿血、痰中的DNA ,结果发现1010例患儿中MP -DNA阳性401例 ,占39.70 % ,其中血液标本阳性率23.60 % ,DNA平均拷贝数7.88×102;痰、咽拭子标本阳性率41.71 % ,DNA平均拷贝数2.71×103。血液与呼吸道标本之间MP -DNA量差异有显著性(P<0.005)。提示FQ -PCR可快速、敏感、准确地定量检测标本中MP -DNA ,了解MP在患儿体内感染和复制情况 ,有助于临床明确诊断与治疗方案的选择
To investigate the early diagnosis of Mycoplasma pneumoniae (MP) infection in children, the DNA in blood and sputum of 1010 children with suspected MP infection was detected by FQ-PCR. The results showed that MP-DNA was positive in 401 children Cases, accounting for 39.70%, of which the positive rate of blood samples 23.60%, the average copy number of DNA 7.88 × 102; sputum, throat swab specimens positive rate of 41.71%, the average copy number of DNA 2.71 × 103. There was a significant difference in MP-DNA between blood and respiratory specimens (P <0.005). Prompt FQ-PCR can be rapid, sensitive and accurate quantitative detection of MP-DNA samples to understand the MP infection and replication in children with the help of clinical diagnosis and treatment options