肺癌细胞p16基因蛋白、RNA和DNA三水平的同步检测研究

• 作 者: 李伟峰 赵彤 邹霞英 朱梅刚
• 机 构: 广州军区广州总医院,第一军医大学
• 出 处: 肿瘤研究与临床
• 发表日期: 1997年04期
• 关键词: 肺癌细胞 基因蛋白 DNA p16基因 肺巨细胞癌细胞 同步检测 RNA 肺肿瘤 肺鳞癌 原位杂交技术

为了解ＭＴＳ１／ｐ１６基因在人肺癌细胞株中的表达情况。应用免疫组化、ＰＣＲ及ＰＣＲ－ＳＳＣＰ与原位杂交技术。发现肺巨细胞癌细胞ｐ１６蛋白表达异常，但ｍＲＮＡ和ＤＮＡ水平检测正常，而肺鳞癌细胞三水平均未检测到异常改变。提示不同类型的肺肿瘤细胞ｐ１６基因表达程度不一致，且ｐ１６基因因翻译水平的调控异常可能参与了ｐ１６基因功能产物的表达调节。

To understand the expression of MTS1/p16 gene in human lung cancer cell lines. Immunohistochemistry, PCR, PCR-SSCP and in situ hybridization were used. It was found that p16 protein expression was abnormal in giant cell lung cancer cells, but normal mRNA and DNA levels were detected, while no abnormal changes were detected in lung squamous cell carcinoma cells. It is suggested that the degree of p16 gene expression in different types of lung tumor cells is inconsistent, and p16 gene may be involved in the expression regulation of p16 gene function product due to the abnormal regulation of translation level.