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本文报道了伯氏鼠疟原虫子孢子侵入体外培养的胚胎鼠脑细胞的数量及其在胚胎鼠脑细胞中发育成不成熟的裂殖体。实验方法是无菌操作解剖按蚊唾液腺,并轻轻压迫唾液腺使之放出子孢子,然后将子孢子集中起来,计算子孢子密度,定量接种于单层胚胎鼠脑细胞,经离心后进行体外培养。培养后,将培养物用布安氏液固定,再用吉氏染液染色检查。7个实验其中有4个实验产生了红外期裂殖体。子孢子感染率为0.01～0.04%,比在体内感染率低2～3级,这可能与子孢子进入脑细胞是人工造成的有关。正常胚胎鼠脑细胞在体外每4天增加一倍,但是当加入感染的或未感染的按蚊唾液腺后,不仅抑制了脑细胞的正常繁殖,而且随着培养时间的延长脑细胞数量逐渐减少。脑
In this paper, we report the number of embryonic rat brain cells invaded by Plasmodium berghei sporozoites in vitro and their development into immature schizonts in embryonic rat brain cells. The experimental method is to dissect the Anopheles salivary gland aseptically and gently press the salivary gland to release the sporozoites. After that, the sporozoites are collected, the sporozoites are counted, and the sporozoites density is quantitatively counted and inoculated into the monolayer embryonic mouse brain cells, centrifuged and cultured in vitro . After culturing, the culture was fixed with Buan’s solution and stained with Kyrgyzstan stain. Four of the seven experiments produced infra-red schizonts. The infection rate of sporozoites is 0.01% -0.04%, which is lower than the infection rate in the body by 2 ~ 3, which may be related to the sporozoites entering the brain cells. Normal embryonic mouse brain cells doubling every 4 days in vitro, but when added to infected or uninfected anopheles salivary gland, not only inhibited the normal reproduction of brain cells, and with the extension of incubation time, the number of brain cells gradually decreased. brain