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将单倍体水稻SAR的幼穗进行愈伤组织的诱导、分化,建立再生体系;并对再生植株进行遗传分析。结果表明:SAR幼穗在组织培养后有加倍现象;倍加单倍体(DH)花粉粒育性良好;籽粒的果皮为红色。微卫星标记结果表明部分DH自交后代是纯合的。纯合单株与无色果皮的二倍体水稻正反交结果表明:F1果皮颜色由母本决定;F2果皮全部为红色;F3出现红色:无色=3:1的分离。初步判定,SAR中含有果皮红色基因;果皮红色性状的遗传是由核内显性单基因控制的,并受母体影响。
The haploid rice SAR young ears callus induction, differentiation, establishment of regeneration system; and regenerated plants for genetic analysis. The results showed that the spikelets of SAR were doubled after tissue culture; the diploid haploid (DH) pollen grains were fertile; the pericarp of the grains was red. Microsatellite markers showed that some DH selfed progeny were homozygous. The results of reciprocal crossing of diploid rice between homozygous plants and colorless peel showed that the color of F1 peel was determined by female parent; F2 peel was all red; F3 appeared red: colorless = 3: 1 separation. It was initially determined that the SAR contained red peel genes. The inheritance of the red peel trait was controlled by a dominant single gene in the nucleus and was influenced by the mother.