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Objective:The aim of our study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed(EVn-50) on the growth of human cervical carcinoma HeLa cells xenografts in nude mice and its possible molecular mechanism.Methods:Models of human cervical cancer HeLa cells xenografts transplanted subcutaneously in nude mice were established and randomly divided into 7 groups(each group including 5 nude mice):saline group,Taxol group,EVn-50 group,comp-6 group,comp-7 group,comp-8 group and comp-10 group.The volume and weight of Xenograts were observed and compared.The alteration of the weight of nude mice,and the change of serum levels of LDH,ALT,Cr and WBC counts were examined and compared.The apoptotic rate of human cervical carcinoma HeLa cells xenografts was analyzed by FCM.The expressions of P53 and Bcl-2 proteins of HeLa cells xenografts were determined by Western blotting.Results:EVn-50 and its fractionated extracts could significantly suppress the increasing volume and weight of human cervical carcinoma HeLa cells xenografts in nude mice models in time-dependent manner,yet had no significant effect on the weight of nude mice,the serum levels of LDH,ALT,Cr and WBC were counted.When the xenografts were treated with EVn-50 and its fractionated extracts for 16 days,the apoptotic rate of xenografts cells were significantly increased,and the expression of P53 protein was up-regulated and protein level of Bcl-2 was decreased.Conclusion:EVn-50 and its fractionated extracts could suppress the growth of human cervical carcinoma HeLa cells xenografts in nude mice,which may be related to its promotion on xenografts cells apoptosis through down-regulation of Bcl-2 expression and activation of P53 expression.
Objective: The aim of The study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed (EVn-50) on the growth of human cervical carcinoma HeLa cells xenografts in nude mice and its possible molecular mechanism. Methods: Models of human cervical cancer HeLa cells xenografts transplanted subcutaneously in nude mice were established and randomly divided into 7 groups (each group including 5 nude mice): saline group, Taxol group, EVn-50 group, comp-6 group, comp- 7 group, comp-8 group and comp-10 group. The volume and weight of Xenograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels of LDH, ALT, Cr and WBC concentrations. and compared. apoptotic rate of human cervical carcinoma HeLa cells xenografts was analyzed by FCM. The expressions of P53 and Bcl-2 proteins of HeLa cells xenografts were determined by Western blotting. Results: EVn-50 and its fractionated extracts could xt ress the increasing volume and weight of human cervical carcinoma HeLa cells xenografts in nude mice models in time-dependent manner, yet had no significant effect on the weight of nude mice, the serum levels of LDH, ALT, Cr and WBC were counted. the xenografts were treated with EVn-50 and its fractionated extracts for 16 days, the apoptotic rate of xenografts cells were significantly increased, and the expression of P53 protein was up-regulated and protein level of Bcl-2 was decreased. Conlusion: EVn- 50 and its fractionated extracts could suppress the growth of human cervical carcinoma HeLa cells xenografts in nude mice, which may be related to its promotion on xenografts cells apoptosis through down-regulation of Bcl-2 expression and activation of P53 expression.