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ＭｃＡｂ－Ｇ３－ＲＩＨＡ诊断血吸虫病、采用绵羊红细胞，经戊二醛固定、鞣酸鞣化，致敏纯化肠相关ＭｃＡｂ，然后冷冻干燥用于检测宿主血清中循环抗原，并以ＩＨＡ对比检测ＣＡｂ。以之检测６３０份样本，其中正常人２２４例，ＲＩＨＡ假阳性率为４．４％，ＩＨＡ为４．９１％；急性血吸虫病１６５例，ＲＩＨＡ阳性率９８．７９、ＩＨＡ９９．３９％；疫区人群２４１份，ＲＩＨＡ阳性率７１．７８％，ＩＨＡ为８４．２３％；其中粪检阳性者ＲＩＨＡ阳性率８６．６７％（６５／７５），粪检阴性者的阳性率是６５．０６％（１０８／１６６），而ＩＨＡ的阳性率分别为９６．０％（７２／７５），７８．９２％（１３１／１６６），ＥＰＧ≥３０的慢性血吸虫病者ＲＩＨＡ及ＩＨＡ阳性率均为１００．０％，当ＥＰＧ＜３０时，ＲＩＨＡ和ＩＨＡ阳性率分别是６９．２３％、９２．３１％。实验结果显示：急性血吸虫病人群循环抗原ＧＭＲＴ为慢性患者的２１．７６倍，不同感染度的慢性患者循环抗原ＧＭＲＴ较为接近。急、慢性血吸虫病血清中ＣＡｂ显著多于循环抗原。本法操作简便，有较好的特异性与敏感性，适用于血吸虫病循环抗原的检测。
McAb-G3-RIHA was used to diagnose schistosomiasis. Sheep red blood cells were fixed by glutaraldehyde and tanned with tannin to sensitize and purify gut-associated McAbs. Lyophilized McAb-G3-RIHA was then used to detect circulating antigens in the host serum and CAb was detected by IHA contrast. To detect 630 samples, of which 224 cases of normal, RIHA false positive rate was 4.4%, IHA was 4.91%; acute schistosomiasis 165 cases, RIHA positive rate was 98.79, IHA99.39%; Among 241 patients, the positive rate of RIHA was 71.78%, while that of IHA was 84.23%. The positive rate of RIHA was 86.67% (65/75), and the positive rate of RIHA was 65.06% The positive rate of IHA was 96.0% (72/75) and 78.92% (131/166) respectively. The positive rates of RIHA and IHA in chronic schistosomiasis patients with EPG≥30 were 100.0 %. When EPG <30, the positive rates of RIHA and IHA were 69.23% and 92.31% respectively. The experimental results showed that GMRT in patients with acute schistosomiasis is 21.76 times of chronic patients, and GMRT of chronic patients with different degrees of infection is relatively close. Serum CAb in acute and chronic schistosomiasis was significantly more than circulating antigen. This method is simple, has good specificity and sensitivity for the detection of circulating antigens in schistosomiasis.