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目的 探讨C57BL/6J小鼠在急性给予喹吡罗（quinpirole, QNP）前后听觉惊跳反射(acoustic startle reflex, ASR)、预先弱刺激抑制(prepulse inhibition, PPI)和空白预先刺激抑制(gap prepulse inhibition, Gap-PPI)的变化情况。方法 20只C57BL小鼠，随机分为实验组(n=10)和阴性对照组(n=10)。实验组给予盐酸QNP腹腔注射，阴性对照组给予等体积生理盐水处理。实验对象均完成惊跳反射的测试。记录注射前的数据为基础值，注射后10min、24h进行相同测试。比较各组惊跳反射强度、预先弱刺激抑制率(%PPI)和空白预先刺激的抑制率(%Gap-PPI)。 结果 对照组小鼠注射生理盐水前后的惊跳反射强度、%PPI和%Gap-PPI未见明显改变（P>0.05）。实验组小鼠注射盐酸QNP 10min后的惊跳反射强度[(0.0307±0.0026)N、%PPI(14.78±6.14)%，%Gap-PPI(17.78±9.74)%]较基础值[(0.0971±0.0043)N，(45.24±10.11)%，(41.49±4.33%)]降低(P<0.05)；实验组小鼠在注射QNP 24h后的惊跳反射强度、%PPI和%Gap-PPI与基础值相比无明显变化（P>0.05）。实验组注射QNP 10min后最大惊跳反射强度、%PPI和%Gap-PPI均较对照组明显降低（P<0.05）。结论 C57BL/6J小鼠急性注射QNP，抑制了预先弱刺激抑制及空白弱刺激抑制，可造成感觉运动门控功能的短期受损并导致小鼠产生耳鸣，可能机制是急性损伤了C57BL/6J小鼠的边缘系统。
Objective To investigate the changes of acoustic startle reflex (ASR), prepulse inhibition (PPI) and gap prepulse inhibition (AFP) in C57BL / 6J mice before and after quinpirole (QNP) , Gap-PPI) changes. Methods Twenty C57BL mice were randomly divided into experimental group (n = 10) and negative control group (n = 10). The experimental group was given intraperitoneal injection of QNP hydrochloride, and the negative control group was given equal volume of normal saline. Subjects completed the reflex test. The data before injection were recorded as the baseline values, and the same test was performed 10 minutes and 24 hours after injection. The levels of reflex intensity, pre-weak stimulation inhibition (% PPI) and blank pre-stimulation inhibition (% Gap-PPI) were compared between groups. Results Before and after injection of saline, the reflex intensity,% PPI and% Gap-PPI of mice in control group showed no significant changes (P> 0.05). Compared with the baseline [(0.0971 ± 0.0043) N,% PPI (14.78 ± 6.14)%,% Gap-PPI (17.78 ± 9.74)%] in the experimental group, ), (45.24 ± 10.11)%, (41.49 ± 4.33%)] (P <0.05). Compared with the baseline values of the experimental group, the reflex intensity,% PPI and% Gap- No significant change (P> 0.05). The maximal reflex intensity,% PPI and% Gap-PPI in experimental group were significantly lower than those in control group after injection of QNP for 10min (P <0.05). Conclusions Acute injection of QNP in C57BL / 6J mice inhibits the inhibition of pre-weak stimulation and the weak stimulation of weak stimulation, which may cause short-term damage of sensory motor gating function and lead to tinnitus in mice. The possible mechanism is acute injury of C57BL / 6J mice Mouse’s edge system.