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目的通过研究Th17细胞和Treg细胞特异性转录因子RORγt和FOXP3在正常妊娠和子痫前期重度患者胎盘组织中的表达,探讨二者在子痫前期发病机制中的作用。方法以子痫前期重度患者35例作为实验组,同期选35例正常妊娠患者作为对照组,用SYBR Green I荧光定量PCR方法,检测胎盘组织中RORγt-mRNA和FOXP3-mRNA的表达水平。结果和对照组相比,子痫前期重度胎盘组织中RORγt-mRNA升高,FOXP3-mRNA的表达降低,RORγt-mRNA/FOXP3-mRNA比值增大(P<0.05);子痫前期重度胎盘组织中RORγt-mRNA与FOXP3-mRNA存在明显的负相关(r=-0.547,P<0.01)。结论促炎性Th17细胞与抑制性Treg细胞之间平衡状态的打破可能是导致子痫前期发病的病因之一。
Objective To investigate the role of Th17 cells and Treg cell-specific transcription factors RORγt and FOXP3 in the placenta of normal pregnancy and severe preeclampsia patients and to explore their roles in the pathogenesis of preeclampsia. Methods 35 patients with severe preeclampsia were selected as experimental group and 35 normal pregnancy patients as control group. The expression of RORγt-mRNA and FOXP3-mRNA in placentas were detected by SYBR Green I quantitative PCR. Results Compared with the control group, the expression of RORγt-mRNA, FOXP3-mRNA and RORγt-mRNA / FOXP3-mRNA were significantly increased in severe preeclampsia placenta (P <0.05) There was a significant negative correlation between RORγt-mRNA and FOXP3-mRNA (r = -0.547, P <0.01). Conclusion The breakdown of the balance between proinflammatory Th17 cells and inhibitory Treg cells may be one of the causes of preeclampsia.