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我们应用特异的抗猪囊尾蚴抗原的单克隆抗体(CCy1),建立了单克隆抗体抑制性ELISA方法以测定囊尾蚴病患者的循环抗原(CAg)。测定灵敏度为ng水平。83例囊尾蚴病患者血清的CAg阳性率为71.1％。其范围为0.16-128μg/ml。对41例囊尾蚴病患者,同时测定了血清和脑脊液中的CAg。单项或两项阳性的总阳性率为90.2％。114例正常人血清、107例其它寄生虫病患者(包括30例包虫病患者)的血清及10例非寄生虫病患者的脑脊液的CAg量均为零。有23例囊尾蚴病患者治疗半年到1年后,有21例抗原浓度为零。另2例分别为0.64μg/ml和1.6μg/ml。表明测定CAg不仅可以确定活动性囊尾蚴感染,而且可考核疗效。
We used a monoclonal antibody specific for anti-Cysticercus cellulosae antigen (CCy1) and established a monoclonal antibody-inhibitory ELISA for the determination of circulating antigen (CAg) in patients with cysticercosis. Determination of sensitivity ng level. The positive rate of CAg in 83 patients with cysticercosis was 71.1%. Its range is 0.16-128 μg / ml. In 41 patients with cysticercosis, serum and cerebrospinal fluid in the determination of CAg. The total positive rate of single or two positives was 90.2%. Cerebrospinal fluid of 114 normal human sera, 107 other parasite disease patients (including 30 echinococcosis patients), and 10 non-parasitic disease patients had a CAg of zero. Twenty-three patients with cysticercosis were treated for six months to one year and 21 had zero antigen concentrations. The other two cases were 0.64μg / ml and 1.6μg / ml respectively. This shows that the determination of CAg can not only determine the activity of Cysticercus infection, but also test the curative effect.