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用 ELISA 法检测了急、慢性肝炎、肝炎后肝硬化患者血清脂蛋白[Lp(a)]水平,现将结果报道如下。资料与方法正常对照组29例,平均年龄62±9.08岁。肝病患者115例,其中肝硬化20例,平均年龄49.5±12岁;急性肝炎50例,平均年龄33.5±5岁;慢性活动性肝炎41例;慢性迁延型肝炎4例,平均年龄40.8±10岁。诊断按1990年上海肝炎会议标准。标本采集:肝硬化组、急肝急性期、慢肝活动期均于首次诊断后次日清晨空腹抽血3ml。急肝恢复期、慢肝稳定期患者临床症状、体征基本消失,ALT、AST、γ-GT 恢复正常后抽血3ml(急肝两次抽血时间间隔30～34天,平均32天,慢肝间隔30～60天,平均45天)。Lp(a)测定用 ELISA 法,试剂盒由南京聚力生物医学工程研究所提供。
The levels of serum lipoprotein [Lp (a)] in patients with acute, chronic hepatitis and posthepatitis cirrhosis were detected by ELISA. The results are reported as follows. Materials and Methods Normal control group of 29 patients, mean age 62 ± 9.08 years old. 115 cases of liver disease, of which 20 cases of cirrhosis, mean age 49.5 ± 12 years; 50 cases of acute hepatitis, the average age of 33.5 ± 5 years; 41 cases of chronic active hepatitis; chronic persistent hepatitis in 4 cases, mean age 40.8 ± 10 years . According to the diagnosis of hepatitis meeting in Shanghai in 1990 standards. Specimen collection: cirrhosis group, acute liver acute phase, slow liver activity were fasting blood 3ml the day after the first diagnosis the next morning. The clinical symptoms and signs disappeared in patients with acute liver recovery and stable phase of chronic liver, blood 3ml was recovered after ALT, AST, and γ-GT returned to normal (twice-daily blood draw time was 30-34 days, with an average of 32 days An interval of 30 to 60 days, an average of 45 days). Lp (a) determination by ELISA method, kit by Nanjing cohesion Biomedical Engineering Institute.