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最近一些肽激素,如人生长激素已在人尿被检测,尿激素排泄可反映24小时收集期血浆的水平,故提供分泌激素有用的资料。作者首次报道人尿类促皮质释放因子免疫反应物(CRF-LI)的存在,因妊娠末3月妇女有高水平CRF-LI,故检查了正常受检者和孕妇的尿标本。 由正常健康受检者(20～40岁,20名)和孕妇(18～36岁,36名)取24小时尿标本,将标本收集在含0.5％硫代水杨酸乙基汞钠,1mM苯基甲基磺酰氟化物和2μg/ml大豆胰蛋白酶抑制剂硅化聚丙烯瓶,在-20℃保存直至分析。用微波炉融化,加合成的碘化人CRF于每一尿标本作“恢复”测定,
Recently, some peptide hormones, such as human growth hormone have been detected in human urine, urinary hormone excretion can reflect the level of plasma collected during the 24-hour period, it provides useful information on the secretion of hormones. The authors first reported the presence of human urine corticogen-releasing factor immunoreactor (CRF-LI). Urine specimens from normal subjects and pregnant women were examined for high levels of CRF-LI in women at the end of the third trimester of pregnancy. Urine specimens were collected from healthy subjects (20-40 years old, 20 women) and pregnant women (18-36 years old, 36 women) for 24 hours. The specimens were collected in 0.5% sodium mercuric thiosalicylate, Phenylmethylsulfonyl fluoride and 2 [mu] g / ml soy trypsin inhibitor Silicone polypropylene bottle, stored at -20 [deg.] C until analysis. Melted with a microwave oven, plus synthetic iodized human CRF in each urine sample for the “recovery” determination,