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几年来的花培实践表明,水稻花粉植株中单倍体的比例一般占30～50％。随着花药培养技术的不断发展,单倍体植株的人工加倍已日益上升成为单倍体育种研究的重要内容之一。由于水稻花粉植株诱导频率较低,加上植株娇嫩细弱,目前尚缺乏高效而又安全的加倍方法。为此,1977年全国花培会议曾把染色体加倍方法的研究列为全国花培八年(1978～1985年)科研规划项目之一。为了探索水稻单倍体高效安全,简便经济的加倍方法,我们自1978年以来进行水稻单倍体再生苗人工加倍研究,现将结果报告如下。
Several years of flower cultivation practice shows that the proportion of haploid rice pollen plants generally account for 30 to 50%. With the continuous development of anther culture technology, doubled haploid plants have been increasingly double artificial haploid breeding an important part of the study. Due to the low induction frequency of rice pollen plants, coupled with delicate plants, there is still no efficient and safe double method. Therefore, in 1977, the national flower cultivation conference once classified the study of chromosome doubling method as one of the research projects of national flower cultivation for eight years (1978-1985). In order to explore the efficient, safe, simple and economical method of rice haploid doubling, we conducted artificial doubling of rice haploid regenerative plants since 1978, and the results are reported as follows.