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目的研究蒙药阿给炭对应激型胃溃疡模型大鼠溃疡组织炎性细胞浸润及新生血管数、微血管密度生成的影响,探讨阿给炭对胃溃疡黏膜下组织重建的作用机制。方法采用水浸束缚应激方法复制急性胃溃疡大鼠模型,实验分为8组,即空白对照组、模型对照组、云南白药组(阳性药)、雷尼替丁组(阳性药)、阿给生药组、阿给炭药低、中、高剂量组。连续给药1周后,常规处死,解剖剪取胃部溃疡组织,HE染色观察炎性细胞浸润,CD34及Ⅷ因子法标记观察溃疡组织微血管密度及新生血管数。结果与正常对照组相比,模型组大鼠溃疡部位新生血管数、微血管密度有显著性差异(P<0.05),与模型组比较,阿给炭高剂量组新生血管数、阿给炭药低剂量组微血管密度有显著性差异(P<0.05)。结论胃溃疡模型大鼠溃疡组织存在新生血管数及微血管密度生成不足,而给予阿给炭治疗后能显著提高溃疡组织新生血管数生成及微血管密度。
Objective To investigate the effect of Mongolian charcoal charcoal on the inflammatory cell infiltration and the number of neovascular vessels and microvessel density in ulcer tissue in rats with gastric ulcer induced by stress, and to explore the mechanism of acai charcoal in the submucosal tissue remodeling of gastric ulcer. Methods The rat model of acute gastric ulcer was established by immersion restraint stress method. The experiment was divided into 8 groups, namely blank control group, model control group, Yunnan Baiyao group (positive drug), ranitidine group (positive drug) To the crude drug group, to charcoal drug low, medium and high dose group. One week after continuous administration, the rats were sacrificed and dissected for gastric ulcer tissue. Infiltration of inflammatory cells was observed by HE staining. Microvessel density and neovascularization of ulcer tissues were observed by CD34 and Ⅷ factor method. Results Compared with the normal control group, the number of neovascular vessels and microvessel density in the model group were significantly different (P <0.05). Compared with the model group, the number of neovascular vessels, There was a significant difference in the microvessel density in the dose group (P <0.05). Conclusions The number of neovascular vessels and the formation of microvessel density in ulcer tissue of gastric ulcer model rats are not enough. However, administration of acacia charcoal can significantly increase the number of neovascularization and microvessel density in ulcer tissue.