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病毒介导的化学敏感基因的转染,为肿瘤的治疗提供了一条有希望的新途径,目前脑肿瘤的基因治疗集中在将包装携带TK基因的逆转录病毒的细胞系植入肿瘤,其主要局限性是逆转录病毒在体内的转染几乎无效,即只有和包装细胞非常接近的胶质瘤细胞才易被转染,且TK基因疗法所产生的旁观者效应需依赖细胞间的直接接触。本文介绍通过复制缺陷的腺病毒载体介导将细菌胞嘧啶脱氨酶(cd)基因转染至大鼠9L胶质瘤细胞,使其对在正常细胞没有毒性的核苷-5-氟胞嘧啶(5-Fc)敏感。
The transfection of virus-mediated chemically sensitive genes provides a promising new approach for the treatment of tumors. Current gene therapy for brain tumors focuses on the packaging of retroviral cell lines carrying the TK gene into tumors. The limitation is that transfection of retroviruses in the body is almost ineffective, that is, only glioma cells that are very close to the packaging cells are easily transfected, and the bystander effect of TK gene therapy depends on direct contact between cells. This article describes the transfection of the bacterial cytosine deaminase (cd) gene into rat 9L glioma cells via replication-defective adenoviral vectors to render it nucleoside-5-fluorocytosine not toxic to normal cells. (5-Fc) sensitive.