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目的:研究CD4+CD25highCD127low/-调节性T细胞(Treg)在胃癌患者外周血中的表达水平,探讨其在肿瘤发病机制及治疗中的作用。方法:采用流式细胞术检测法检测90例胃癌患者(35例为早中期胃癌,55例为晚期胃癌)和30名健康体检者外周血中CD4+CD25highCD127low/-Treg的表达水平。结果:90例胃癌患者外周血CD4+T细胞中CD4+CD25highCD127low/-Treg含量为(11.60±5.99)%,高于健康体检者(5.19±1.72)%,t=5.610,P=0.007;Ⅲ+Ⅳ期患者外周血CD4+T细胞中CD4+CD25high CD127low/-Treg含量为(12.55±6.59)%,明显高于Ⅰ+Ⅱ期患者(10.39±4.68)%(t=4.113,P=0.04)和健康体检者(5.19±1.72)%(t=5.923,P=0.001)。手术后胃癌患者CD4+CD25highCD127low/-Treg水平为(6.12±2.13)%,明显低于手术前(11.25±5.63)%,t=5.237,P=0.04。结论:胃癌患者外周血CD4+CD25highCD127low/-Treg数量增多且与病程分期相关,手术后Treg数量明显减少,Treg可能参与了胃癌的发生发展。
Objective: To investigate the expression of CD4 + CD25highCD127low / - regulatory T cells (Tregs) in peripheral blood of patients with gastric cancer and to explore its role in the pathogenesis and treatment of cancer. Methods: Flow cytometry was used to detect the expression of CD4 + CD25highCD127low / -Treg in peripheral blood of 90 patients with gastric cancer (35 with early gastric cancer, 55 with advanced gastric cancer) and 30 healthy controls. Results: The percentage of CD4 + CD25highCD127low / -Treg in CD4 + T cells from peripheral blood of 90 patients with gastric cancer was (11.60 ± 5.99)%, higher than 5.19 ± 1.72%, t = 5.610, P = 0.007, The level of CD4 + CD25high CD127low / -Treg in peripheral blood CD4 + T cells in stage Ⅳ patients was (12.55 ± 6.59)%, significantly higher than that in stage Ⅰ + Ⅱ patients (10.39 ± 4.68)% (t = 4.113, P = 0.04) Health examination (5.19 ± 1.72)% (t = 5.923, P = 0.001). The level of CD4 + CD25highCD127low / -Treg in postoperative gastric cancer patients was (6.12 ± 2.13)%, significantly lower than that before operation (11.25 ± 5.63)%, t = 5.237, P = 0.04. CONCLUSION: The number of CD4 + CD25highCD127low / -Tregs in peripheral blood of patients with gastric cancer is increased and correlated with the stage of the disease. The number of Tregs after operation is significantly reduced, and Tregs may be involved in the occurrence and development of gastric cancer.