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目的:以粪肠球菌为研究对象,探讨粪肠球菌基因srtA(转肽酶A编码基因)、esp(肠球菌表面蛋白)与粪肠球菌生物被膜形成早期的相关性。方法:用逆转录PCR与实时荧光定量PCR方法对生物被膜和浮游菌组细菌srtA、esp两种与生物被膜形成早期相关的基因其表达进行检测,并进行统计学分析。结果:srtA、esp基因与粪肠球生物被膜菌早期形成密切相关。生物被膜菌组srtA、esp表达量分别是浮游菌组的7.9与13.5倍。结论:srtA、esp基因与粪肠球生物被膜菌形成早期密切相关,可能是生物被膜早期形成的上调因子。
Objective: Enterococcus faecalis was used as research object to investigate the early correlation of Enterococcus faecalis srtA (transpeptidase A gene), esp (enterococcal surface protein) and Enterococcus faecalis biofilm formation. Methods: The expressions of srtA and esp, two early genes related to biofilm formation in biofilms and planktonic bacteria, were detected by RT-PCR and real-time fluorescence quantitative PCR. Statistical analysis was performed. Results: srtA and esp genes were closely associated with the early formation of Enterococcus faecalis biofilm. The biofilm group srtA, esp expression levels were 7.9 and 13.5 times the planktonic group. Conclusion: The srtA and esp genes are closely related to the formation of fecal enterococci in the early stage, which may be the early up-regulation of biofilm formation.