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目的研究膀胱癌癌细胞株T24的VEGF、TGF-β1分泌与TLR2信号通路的关系。方法采用实时荧光定量RT-PCR检测膀胱癌细胞株T24的TLR2表达情况;使用实时荧光定量RT-PCR和ELISA检测经TLR2激活物肽聚糖处理前后癌细胞VEGF及TGF-β1 mRNA的水平及蛋白质分泌状况;使用实时荧光定量RT-PCR和ELISA检测采取抗TLR2抗体封闭后再用肽聚糖处理前后肿瘤细胞VEGF和TGF-β1 mRNA的水平及蛋白质分泌状况。结果膀胱癌细胞株T24膜上有TLR2表达;经TLR2激活物肽聚糖处理后癌细胞VEGF及TGF-β1 mRNA的水平显著增高,在细胞培养上清液中蛋白质分泌水平同样显著增高;在采用抗TLR2抗体封闭TLR2后,肽聚糖的刺激不会引起癌细胞VEGF和TGF-β1 mRNA的水平及蛋白质分泌水平的增高。结论 TLR2信号通路促进VEGF和TGF-β1分泌参与膀胱癌细胞免疫逃逸,这对预防、诊断和治疗膀胱癌可能会发挥重要作用。
Objective To study the relationship between the secretion of VEGF and TGF-β1 in bladder cancer cell line T24 and the TLR2 signaling pathway. Methods The expression of TLR2 in bladder cancer cell line T24 was detected by real-time fluorescence quantitative RT-PCR. The levels of VEGF and TGF-β1 mRNA and protein in cancer cells before and after TLR2 activator peptidoglycan treatment were detected by real-time fluorescence quantitative RT-PCR and ELISA Secretion and the secretion of VEGF and TGF-β1 mRNA before and after treatment with anti-TLR2 antibody by using real-time fluorescence quantitative RT-PCR and ELISA. Results The expression of TLR2 on bladder cancer cell line T24 was significantly increased. The levels of VEGF and TGF-β1 mRNA in cancer cell lines treated with TLR2-activated peptidoglycan were significantly increased, and the secretion of protein in cell culture supernatant was also significantly increased. Anti-TLR2 antibody blocking TLR2, peptidoglycan stimulation will not cause cancer cells VEGF and TGF-β1 mRNA levels and protein secretion increased. Conclusion The TLR2 signaling pathway promotes the secretion of VEGF and TGF-β1 in bladder cancer cells, which may play an important role in the prevention, diagnosis and treatment of bladder cancer.