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树突状细胞(DC)是免疫系统中一类重要的抗原提呈细胞(APC),抗原提呈功能最强,其特点是能激发静息的T细胞产生免疫应答.在树突状细胞的发育过程中,粒细胞-巨噬细胞集落刺激因子(GM-CSF),具有关键作用.近来文献报道,GM-CSF与IL-4或TNF联合应用,可使外周血中的CD14阳性单核细胞分化为树突状细胞.提示树突状细胞可来源于单核细胞.目前,关于诱导肿瘤细胞分化为树突状细胞的研究,尚未见文献报道.我们曾初步观察到GM-CSF处理后的FBL-3细胞,单核细胞的特异性标志CD14表达水平显著增加,NBT还原能力增强,提示GM-CSF可诱导小鼠FBL-3红白血病细胞分化为单核细胞.
Dendritic cells (DCs) are an important class of antigen-presenting cells (APCs) in the immune system. They have the highest antigen-presenting capacity and are characterized by their ability to stimulate resting T cells to produce immune responses. In dendritic cells During development, granulocyte-macrophage colony stimulating factor (GM-CSF) plays a key role. Recently, it has been reported that GM-CSF combined with IL-4 or TNF can make CD14 positive monocytes in peripheral blood. Differentiation into dendritic cells suggests that dendritic cells can be derived from monocytes. At present, studies on the induction of differentiation of tumor cells into dendritic cells have not been reported in the literature. We have initially observed that after GM-CSF treatment FBL-3 cells, monocyte-specific markers of CD14 expression was significantly increased, NBT reduced ability to increase, suggesting that GM-CSF can induce mouse FBL-3 erythroleukemia cells into mononuclear cells.