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作者用减毒曼氏血吸虫尾蚴接种的小鼠和正常的小鼠,经尾蚴攻击感染后,用吡喹酮治疗,观察疗效。试验用雌性CBA/Ca小鼠,体重18～22g。曼氏血吸虫波多黎各株在实验室用光滑双脐螺(Biomphalaria glabrat)和金色仓鼠传代。制备1000条/ml尾蚴悬液,用20K的Υ射线照射1小时,作为接种液。以30mg/kg戊巴比妥钠经腹膜内注射麻醉小鼠,按Smithers方法用约500条照射减毒的尾蚴进行接种。4周后,按上述方法麻醉接种小鼠和正常小鼠(接种组和对照组每组均为6只),
The authors inoculated with attenuated Schistosoma mansoni cercariae mice and normal mice, infected with cercariae infection, with praziquantel treatment to observe the effect. Female CBA / Ca mice tested weighing 18-22 g. S. mansoni Puerto Rico strains were passaged in labs using the biomphalaria glabrat and golden hamster. A suspension of 1000 pieces / ml of cercariae was prepared and irradiated with Υ-rays of 20K for 1 hour as inoculum. Mice were anesthetized with 30 mg / kg sodium pentobarbital via intraperitoneal injection and inoculated with about 500 attenuated cercariae according to the Smithers method. Four weeks later, the mice inoculated and the normal mice were anesthetized as above (6 in each group in inoculation group and control group)