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目的 :探讨免疫组织化学S_P微波法对阴性淋巴结微转移灶的检出率。方法 :采用S_P微波法 ,对 30例舌鳞状细胞癌患者既往报告为阴性的 15 2个淋巴结的石蜡标本再作切片 ,并作免疫组织化学染色。取阳性淋巴结作为对照。结果 :30例患者 15 2个阴性淋巴结 ,经CK(AE1/AE3)单克隆抗体标记后 ,在 4例 (13 3% )患者中共 7个淋巴结 (4 6 % )内CK(AE1/AE3)单克隆抗体呈阳性反应。癌细胞呈单个或呈簇状散在于淋巴结内 ,淋巴结结构完好。检出的 4例患者 ,高分化及低分化鳞状细胞癌各 2例 ,其中 2例术前已发现颈部有肿大淋巴结 ,7个微转移之淋巴结中 ,颌下区 2 ,颈深上 4 ,颈深中 1。正常淋巴结呈阴性反应。结论 :舌癌患者的阴性淋巴结中 ,仍有一定数量的微转移灶发生 ,采用免疫组化的方法 ,对发现与诊断微转移灶具有一定的可靠性和应用前景
Objective: To investigate the detection rate of negative lymph node micrometastasis by immunohistochemical S_P-microwave method. Methods: S_P microwave method was used to slice and paraffin-embedded paraffin-embedded specimens from 15 cases of tongue squamous cell carcinoma in 15 2 lymph nodes, which were negative in the past. Immunohistochemical staining was also performed. Positive lymph nodes were taken as controls. Results: Fifty-two negative lymph nodes in 30 patients were labeled with CK (AE1 / AE3) monoclonal antibody (CK1) in 7 lymph nodes (46%) of 4 patients Clonal antibody was positive. Cancer cells were single or cluster scattered in the lymph nodes, lymph node structure intact. Four cases were detected, 2 cases were highly differentiated and poorly differentiated squamous cell carcinoma, 2 cases had enlarged cervical lymph nodes before operation, 7 subtasal lymph nodes, 2 submandibular neck, 4, deep in the neck Normal lymph nodes were negative. Conclusion: There is still a certain number of micrometastases in the negative lymph nodes of tongue cancer patients. Immunohistochemical method is used to detect and diagnose micrometastases with certain reliability and application prospect