论文部分内容阅读
目的比较慢性乙型肝炎病毒(hepatitis B virus,HBV)感染不同临床转归患者外周血自然杀伤(NK)细胞程序性凋亡因子-1(programmed death-1,PD-1)的表达情况。方法流式细胞术检测30例健康者(对照组)、17例慢性HBV携带者(携带组)、53例慢性肝炎者(慢性肝炎组)、18例肝炎肝硬化患者(肝硬化组)、16例乙肝相关原发性肝癌者(肝癌组)外周血NK细胞PD-1表达率,并进行比较。结果对照组NK细胞PD-1表达率[(8.2±2.0)%]明显低于携带组[(15.9±5.6)%]、肝硬化组[(14.8±6.8)%]和肝癌组[(14.8±4.1)%],差异有统计学意义(P=0.000)。对照组与慢性肝炎组[(10.6±4.5)%]相比,差异无统计学意义(P=0.258)。4组慢性HBV感染不同临床转归组NK细胞PD-1表达率比较,仅携带组NK细胞PD-1表达率高于慢性肝炎组,差异有统计学意义(P=0.001)。结论 NK细胞高表达PD-1可能影响机体对HBV感染的清除。
Objective To compare the expression of programmed death-1 (PD-1) in peripheral blood of patients with chronic hepatitis B virus (HBV) infection. Methods Thirty healthy controls (control group), 17 chronic HBV carriers (carrier group), 53 chronic hepatitis patients (chronic hepatitis group), 18 hepatitis cirrhosis patients (cirrhosis group) and 16 Cases of hepatitis B-related primary liver cancer (liver cancer group) peripheral blood NK cell PD-1 expression rates, and compared. Results The expression of PD-1 in control group was significantly lower than that in the control group [(8.2 ± 2.0)% vs [(15.9 ± 5.6)% vs 14.8 ± 6.8% 4.1%], the difference was statistically significant (P = 0.000). The difference between the control group and the chronic hepatitis group [(10.6 ± 4.5)%] was not statistically significant (P = 0.258). The expression of PD-1 in NK cells of four groups with chronic HBV infection was significantly higher than that in chronic hepatitis group (P = 0.001). Conclusion High expression of PD-1 on NK cells may affect the clearance of HBV infection in the body.