论文部分内容阅读
应用敏感的单克隆抗体捕获夹心法酶联免疫吸附试验,检测202例各型HBV感染者血清IgA抗-HBc.在1∶1000稀释度,重肝、肝硬化、慢活肝、急乙肝、肝癌、慢迁肝和无症状HBsAg携带者IgA抗-HBc阳性率分别为100%、89.47%、89.19%、87.5%、85%、77.5%、和67.5%在1∶5000稀释度,急乙肝的S/N值(X±SD)是7.59±3.40,显著高于慢活肝(5.62±3.93)、慢迁肝(5.05±3.19)和无症状HBsAg携带者(1.99±2.74)(P<0.05).结果提示IgA抗-HBc与肝损害程度密切相关;对急乙肝的诊断和预后判断有一定意义;多数无症状HBsAg携带者有肝损害.
Sensitive monoclonal antibody capture sandwich enzyme-linked immunosorbent assay was used to detect serum IgA anti-HBc in 202 patients with various types of HBV infection.At 1: 1000 dilution, severe hepatitis, cirrhosis, slow-living liver, acute hepatitis B, liver cancer , The positive rates of IgA anti-HBc in slow-moving liver and asymptomatic HBsAg carriers were 100%, 89.47%, 89.19%, 87.5%, 85%, 77.5%, and 67.5% at 1: 5000 dilution, respectively / N value (X ± SD) was 7.59 ± 3.40, which was significantly higher than that of slow-living liver (5.62 ± 3.93), slow-moving liver (5.05 ± 3.19) and asymptomatic HBsAg carriers (1.99 ± 2.74) (P <0.05). The results suggest that anti-HBc IgA is closely related to the degree of liver damage; diagnosis and prognosis of acute hepatitis B have a certain significance; most of the asymptomatic HBsAg carriers have liver damage.