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小麦花药培养,旨在利用单倍体植株加倍后的纯合性,缩短杂交育种时间,随着“花培一号”、“京花一号”、“豫花一号”等小麦新品种的育成,无疑单倍体育种是小麦育种的新途径之一,但目前小麦花药培养在诱导愈伤组织和分化绿苗频率仍很低。本文研究了不同试验材料,不同培养基及试验材料采前灌水对诱导愈伤组织和分化绿苗频率的影响,为提高诱导愈伤组织和分化绿苗频率提供理论依据。
The anther culture of wheat aims to shorten the breeding time by doubling the homozygosity of the haploid plants. With the development of new varieties of wheat such as “Hupei No.1”, “Jinghua No.1” and “Yuhua No.1” Bred, undoubtedly haploid breeding is one of the new ways of wheat breeding, but the current wheat anther culture in callus induction and differentiation of green shoots is still very low frequency. In this paper, the effects of different experimental materials, different culture media and experimental materials on the induction of callus and differentiation of green plantlets were studied in order to provide theoretical basis for increasing the frequency of inducing callus and differentiation of green plantlets.