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目的 探讨乙型肝炎患者病毒前 S1抗原与病毒 DNA的关系。方法 对 1 86例乙型肝炎大三阳患者分别进行前 S1抗原和 DNA检测 ,前 S1用酶联免疫法 ,DNA用蛋白印迹法。结果 两法的阳性检出率分别为 95 .2 %和88.2 % ,前 S1抗原法检出率略高 (RRR>-2 5 % ) ;两法检测结果呈变量关系 (γ=0 .0 3 8,P >0 .0 5 )。结论 作为反映乙肝病毒复制和传染性的血清学标志 ,前 S1抗原和 DNA与 HBe Ag相一致 ,与非 PCR DNA法比较 ,前 S1抗原法灵敏度稍高 ,可以作为非 PCR DNA检测法的加强和补充
Objective To investigate the relationship between viral S1 DNA and viral DNA in hepatitis B patients. Methods One hundred and seventy-six patients with hepatitis B and Sansheng were tested for pre-S1 antigen and DNA, the former S1 enzyme-linked immunosorbent assay and the Western blotting. Results The positive detection rates of the two methods were 95.2% and 88.2%, respectively. The detection rate of the pre-S1 antigen method was slightly higher (RRR> -2.5%). The results of the two methods showed a variable relationship (γ = 0.30 8, P> 0. 05). Conclusion As a serological marker reflecting hepatitis B virus replication and infectivity, the pre-S1 antigen and DNA are consistent with HBe Ag. Compared with non-PCR DNA method, the pre-S1 antigen method has a slightly higher sensitivity and can be used as an enhancement and supplement