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应用核酸分子杂交技术对雌雄异株植物山靛几个性别类型生殖器官细胞质poly(A)+RNA进行了复性动力学的研究,结果表明同,雄性不育系、雄性恢复系和雄性可育系相比,poly(A)+RNA碱基数和序列种数均以恢复系为高,不育系次之,雄性可育系最低,异源杂交结果表明,各系均有自己独特的序列,不育系与恢复系的序列差异,主要位于中度重复序列和单拷贝序列类:不育系与可育系的差异,则从高度重复序列开始就表现出来,且这种差异一直持续到中度重复序列类和单拷贝序列,恢复系与可育系相比,虽然其表现型一致,但它们具有不同的poly(A)+RNA群体,表明二者在信使核糖核酸转录与加工过程中,仍然存在着差异。
The study of the refolding kinetics of poly (A) + RNA in several sex genotypes of genital organs of dioecious plants by nucleic acid molecular hybridization showed that the male sterile, male restorer and male fertile The number of poly (A) + RNA bases and the number of sequences were all higher in restorer lines, followed by male sterility lines, and lowest in male fertile lines. The result of heterologous hybridization showed that each line had its own unique sequence , The sequence differences between male sterile and restorer lines are mainly located in moderate and single copy sequences: the difference between male sterile line and male sterile line appears from the highly repeatable sequence, and this difference lasted until Median repeats and single-copy sequences, which have different poly (A) + RNA populations compared to fertile lines, have identical polymolecular phenotypes, indicating that they are involved in the transcription and processing of messenger ribonucleic acids There are still differences.