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提出一种针对生物体共焦层析图象的三维重建算法，在荧光激发数学模型的基础上研究定向光线在空间中的传输，逐层递推得到任意方向上的消隐视图。该算法可较好重现试样的半透明效果，根据需要调整参数可使同一试样呈现透明、半透明或不透明的外观，辅助定向光源的引入有助于突出深层组织的细节结构，且具有其它算法无法比拟的运算速度。给出了算法的实现及实例。
A three-dimensional reconstruction algorithm for biofocus confocal tomography image is proposed. Based on the fluorescence excitation mathematical model, the directional light transmission in space is studied, and the blanking view in any direction is obtained by layer-by-layer recursion. The algorithm can reproduce the translucent effect of the sample better. The parameters can be adjusted as needed to make the same sample appear transparent, translucent or opaque appearance. The introduction of the auxiliary directional light source helps to highlight the detailed structure of deep tissue and has Other algorithms can not be compared to the speed of operation. The realization of the algorithm and examples are given.