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目的：探讨谷氨酰胺（ＧＬＮ）对缺氧复氧损伤人小肠上皮细胞（ＩＥＣ）内还原型谷胱甘肽（ＧＳＨ）和丙二醛（ＭＤＡ）的影响。方法：采用原代培养人ＩＥＣ作为实验模型，用荧光法测定体外培养ＩＥＣ内ＧＳＨ和ＭＤＡ含量。结果：正常对照组ＧＳＨ含量最高，在对照组加入ＧＬＮ并不影响ＧＳＨ含量（Ｐ＞０．０５）。缺氧６０ｍｉｎ或９０ｍｉｎ复氧３０ｍｉｎ损伤ＩＥＣ细胞内ＧＳＨ含量均显著低于正常对照组（Ｐ＜０．０５），预先应用ＧＬＮ可显著阻止缺氧复氧损伤ＩＥＣ细胞ＧＳＨ含量下降（Ｐ＜０．０５）。相反，正常对照组ＭＤＡ含量最低，缺氧６０ｍｉｎ复氧３０ｍｉｎＩＥＣ细胞内ＭＤＡ水平显著高于正常对照组（Ｐ＜０．０１），预先应用ＧＬＮ可显著降低缺氧复氧损伤ＩＥＣ细胞内ＭＤＡ水平（Ｐ＜０．０５）。结论：补充ＧＬＮ可维持缺氧复氧损伤ＩＥＣ细胞ＧＳＨ水平，减轻细胞膜脂质过氧化
AIM: To investigate the effect of glutamine (GLN) on glutathione (GSH) and malondialdehyde (MDA) in human intestinal epithelial cells (IEC) induced by hypoxia and reoxygenation. Methods: Primary cultured human IEC was used as experimental model, and the content of GSH and MDA in IEC cultured in vitro was measured by fluorescence method. Results: The content of GSH in the normal control group was the highest. The addition of GLN in the control group did not affect the content of GSH (P> 0.05). The level of GSH in IEC cells exposed to hypoxia and reoxygenation for 30min was significantly lower than that of normal control group (P <0.05). Pretreatment with GLN significantly inhibited the decrease of GSH level in IEC cells exposed to hypoxia / reoxygenation injury (P <0.05). 05). On the contrary, the content of MDA in the normal control group was the lowest, the level of MDA in 30min IEC reoxygenation was significantly higher than that in the normal control group (P <0.01) at 60min hypoxia, GLN pretreatment could significantly reduce the MDA level P <0.05). Conclusion: Supplementation of GLN can maintain the level of GSH in hypoxic and reoxygenation-damaged IEC cells and reduce the lipid peroxidation