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目的:研究黄芩与五味子配伍对黄芩苷和五味子酯甲的大鼠药代动力学规律的影响。方法:大鼠灌胃黄芩提取物2.5 g.kg-1﹑五味子提取物2.5 g.kg-1和黄芩提取物加五味子提取物各2.5 g.kg-1,分别于0.25,1,2,4,8,12,24 h取血分离血浆,采用HPLC测定其黄芩苷﹑五味子酯甲的含量,结果运用DAS 3.0计算其药动学参数。结果:测定黄芩组黄芩苷的药动学参数分别为t1/2=(6.203±3.324)h,AUC=(41.868±28.254)μg.L-1.h-1,Cmax=(2.883±0.684)μg.L-1,MRT=(11.697±4.108)h,Vd=(568 112.69±81 364.658)L.kg-1,CL=(98 526.569±93 774.892)L.h-1.kg-1;黄芩+五味子组黄芩苷的药动学参数分别为t1/2=(10.686±1.533)h,AUC=(53.064±30.047)μg.L-1.h-1,Cmax=(3.168±1.312)μg.L-1,MRT=(16.147±1.79)h,Vd=(2 240 724.1±1 824 718.9)L.kg-1,CL=(139 855.27±107 995.59)L.h-1.kg-1;黄芩+五味子组五味子酯甲的药动学参数分别为t1/2=(21.544±14.611)h,AUC=(11.554±5.516)μg.L-1.h-1,Cmax=(0.311±0.074)μg.L-1,MRT=(34.139±18.532)h,Vd=(12 153 917±5 806 489.8)L.kg-1,CL=(564 758.71±384 128.86)L.h-1.kg-1;五味子组五味子酯甲的药动学参数分别为t1/2=(4.926±5.371)h,AUC=(4.988±3.029)μg.L-1.h-1,Cmax=(0.287±0.071)μg.L-1,MRT=(12.002±6.854)h,Vd=(3 091.656±1 585.602)L.kg-1,CL=(615.571±250.643)L.h-1.kg-1。结论:黄芩与五味子配伍,可以促进黄芩苷和五味子酯甲在血液中的吸收,并可延长这两种成分的半衰期,使其血药浓度维持在较高水平,达到协同增效的目的。
Objective: To study the effects of the compatibility of Astragalus membranaceus and Schisandra chinensis on the pharmacokinetics of baicalin and schisandrin A in rats. METHODS: Rats were treated with 2.5 g.kg-1 Astragalus membranaceus extract, 2.5 g.kg-1 Schisandra chinensis extract, and 2.5 g.kg-1 each of Astragalus membranaceus extract and schisandra extract, respectively, at 0.25, 1, 2, 4 After 8 hours, 12 hours, and 24 hours, blood was collected to separate plasma. The contents of baicalin and schisandrin A were determined by HPLC, and the pharmacokinetic parameters were calculated using DAS 3.0. Results: The pharmacokinetic parameters of baicalin in the astragalus group were determined as t1/2=(6.203±3.324)h, AUC=(41.868±28.254)μg.L-1.h-1, Cmax=(2.883±0.684)μg. .L-1, MRT = (11.697 ± 4.108) h, Vd = (568 112.69 ± 81 364.658) L.kg-1, CL = (98 526.569 ± 93 774.892) Lh-1.kg-1; Astragalus + Schisandrae group The pharmacokinetic parameters of baicalin were t1/2=(10.686±1.533)h, AUC=(53.064±30.047)μg.L-1.h-1, and Cmax=(3.168±1.312)μg.L-1, respectively. MRT = (16.147 ± 1.79) h, Vd = (2 240 724.1 ± 1 824 718.9) L.kg-1, CL = (139 855.27 ± 107 995.59) Lh-1.kg-1; Astragalus + Schisandra schisandrae A The pharmacokinetic parameters were t1/2=(21.544±14.611)h, AUC=(11.554±5.516) μg.L-1.h-1, Cmax=(0.311±0.074)μg.L-1, MRT=, respectively. (34.139±18.532)h, Vd=(12 153 917±5 806 489.8) L.kg-1, CL=(564 758.71±384 128.86)Lh-1.kg-1; Pharmacokinetics of Schisandra chinensis A of Wuweizi Formation The parameters were t1/2=(4.926±5.371)h, AUC=(4.988±3.029) μg.L-1.h-1, Cmax=(0.287±0.071) μg.L-1, MRT=(12.002±6.854). h,Vd=(3 091.656±1 585.602) L.kg-1, CL=(615.571±250.643)Lh-1.kg-1. Conclusion: The compatibility of Astragalus membranaceus with Schisandra chinensis can promote the absorption of baicalin and schisandrin A in blood, and prolong the half-life of these two components, so as to maintain the plasma concentration at a high level and achieve synergistic effects.