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A novel electrochemical immunoassay for cardiac troponin I (cTnI) combining theconcepts of the dual monoclonal antibody “sandwich” principle, the silver enhancement on thenano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode (SBA-MCPE) isdescribed. Four main steps were carried out to obtain the analytical signal, i.e., electrodepreparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection.A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.
A novel electrochemical immunoassay for cardiac troponin I (cTnI) combining the concepts of the dual monoclonal antibody “sandwich” principle, the silver enhancement on thenano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode (SBA-MCPE) isdescribed. Four main steps were carried out to obtain the analytical signal, ie, electrodepreparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng / mL and a limit of detection of 0.2 ng / mL of cTnI were obtained.