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目的:了解脂肪乳剂预处理后对急性肺损伤(ALI)大鼠肺系数和肺组织病理形态的影响。方法:幼年雄性大鼠100只,随机分为五组,分别为对照(blank)组、内毒素(LPS)组、Inralipid(Intra)组、Clinoleic(Cli-no)组和Omegaven(Omega)组,每组20只。五组大鼠分别用等渗盐水或3种脂肪乳剂行肠外营养(PN)7 d。blank组大鼠给予气管内滴入等渗盐水,其余四组滴入LPS,建立ALI大鼠模型。8 h后,取大鼠肺组织并称重,行H-E染色和TUNEL检测。结果:ALI模型组大鼠的肺系数、病理评分和细胞凋亡指数均显著高于blank组(P<0.05或P<0.01);ALI模型组大鼠均有不同程度的肺间质水肿、出血,炎性细胞浸润,凋亡细胞较多。采用PN的大鼠病理评分均显著轻于LPS组(P<0.05或P<0.01);Clino组和Omega组的凋亡细胞指数显著低于LPS组(P<0.05或P<0.01)。结论:脂肪乳剂能减轻ALI大鼠肺组织的病理改变,Clinoleic和Omegaven还能显著减少细胞凋亡。
Objective: To investigate the effect of fat emulsion pretreatment on lung coefficient and lung histopathology in rats with acute lung injury (ALI). Methods: 100 young male rats were randomly divided into five groups: blank group, LPS group, Inralipid (Intra) group, Clinoleic (Cli-no) group and Omegaven (Omega) Each group of 20. Five groups of rats were given parenteral nutrition (PN) with isotonic saline or three kinds of fat emulsion for 7 days respectively. The rats in blank group were given intra-tracheal instillation of isotonic saline, and the remaining four groups were instilled with LPS to establish ALI rat model. After 8 h, the lungs of rats were weighed, H-E staining and TUNEL assay were performed. Results: The lung index, pathological score and apoptotic index in ALI model group were significantly higher than those in blank group (P <0.05 or P <0.01). ALI model rats had different degrees of interstitial edema and hemorrhage , Inflammatory cell infiltration, apoptotic cells more. The pathological score of PN rats was significantly lower than that of LPS group (P <0.05 or P <0.01). The apoptotic index of Clino group and Omega group was significantly lower than that of LPS group (P <0.05 or P <0.01). Conclusion: Fat emulsion can reduce the pathological changes of lung tissue in ALI rats. Clinoleic and Omegaven can also significantly reduce apoptosis.