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目的探讨IL-6启动子-174G/C和-572C/G的单核苷酸多态性频率分布与2型糖尿病患者下肢血管病变的关系。方法 280例中国北方地区汉族成年2型糖尿病患者,根据临床诊断分为下肢血管病变(VDLE)组、下肢神经病变(NDLE)组、下肢血管-神经病变(VNDLE)组和无下肢并发症(NC)组,另选70例社区查体正常成人作为对照(CON)组。采用PCR-RFLP法测定IL-6启动子区-174G/C和-572C/G单核苷酸多态性;采用ELISA法测定血清IL-6、IL-6受体和hsCRP。结果 IL-6启动子的-174G/C基因型及等位基因在2型糖尿病组和对照组的频率分布差异均无统计学意义。而IL-6启动子-572C/G在2型糖尿病组中的基因型GG和CG、等位基因G均高于对照组(P<0.05),并且VDLE组和VNDLE组高于其他组(P<0.05)。结论 2型糖尿病下肢血管病变与IL-6启动子的-174G/C单核苷酸多态性可能无关,而与IL-6启动子区-572C/G单核苷酸多态性可能有关。IL-6启动子区-572C/G的GG基因型和G等位基因可能是2型糖尿病发生下肢血管病变的易患基因。
Objective To investigate the relationship between IL-6 promoter-174G / C and -572C / G single nucleotide polymorphism (SNP) frequency and lower extremity vascular lesions in type 2 diabetic patients. Methods A total of 280 Han adults with type 2 diabetes mellitus in northern China were divided into two groups based on clinical diagnosis: VDLE, NDLE, VNDLE and NC ) Group, another 70 cases of community physical examination of normal adults as a control (CON) group. IL-6 promoter -174G / C and -572C / G SNPs were detected by PCR-RFLP. Serum IL-6, IL-6 receptor and hsCRP were measured by ELISA. Results There was no significant difference in the frequency distribution of -174G / C genotype and allele between IL-6 promoter and control group in type 2 diabetes mellitus group. The genotype GG, CG and allele G of IL-6 promoter-572C / G in type 2 diabetes mellitus group were higher than those in control group (P <0.05), and were higher in VDLE group and VNDLE group than in other groups <0.05). Conclusion The lower extremity vascular lesions in type 2 diabetes may not be related to the -174G / C SNP of IL-6 promoter, but may be related to the -572C / G SNP in IL-6 promoter. The GG genotype and G allele of -572C / G in the IL-6 promoter region may be susceptible to lower extremity vascular disease in type 2 diabetes mellitus.