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用循环伏安法考察了二茂钛二甲硫氨酸配合物(TMC)与DNA在pH=3.0的KCl水溶液中相互作用的电化学行为。TMC在玻碳电极上分别于-412 mV和-528 mV(υs.SCE)有一个氧化峰和相应的还原峰。加入DNA后,TMC的式量电位负移5 mV,峰电流增加,电化学参数(α和ks)改变。结果表明:在该条件下,TMC与DNA之间通过静电作用,形成了一种具有电活性的复合物,且TMC与DNA之间的结合位点数n和结合常数K分别为4.36和2.74×1016。结合紫外-可见光谱法测定结果,加入DNA后TMC的紫外-可见光谱在393 nm处呈现增色效应,进一步确证了TMC与DNA之间主要以静电作用为主。
Cyclic voltammetry was used to investigate the electrochemical behavior of the interaction between titanocene-dimethyl-methionine complex (TMC) and DNA in aqueous KCl at pH = 3.0. TMC has an oxidation peak and a corresponding reduction peak at -412 mV and -528 mV (υs.SCE) at the glassy carbon electrode. After addition of DNA, the potential of TMC was negatively shifted by 5 mV, the peak current increased, and the electrochemical parameters (α and ks) changed. The results showed that under this condition, an electrocompetent complex was formed between TMC and DNA through electrostatic interaction, and the number of binding sites n and binding constant K between TMC and DNA were 4.36 and 2.74 × 1016, respectively . Combined with UV-Vis spectrophotometry, UV-vis spectra of TMC showed an enhancement effect at 393 nm after addition of DNA, which further confirmed that the electrostatic interaction between TMC and DNA was the main one.