论文部分内容阅读
实验在给予慢性应激刺激的SpapeDawlay雄性大鼠中进行，用核酸斑点杂交技术观察大鼠下丘脑组织中血管升压汞（AVP）mRNA水平变化，用异羟基洋地黄毒甙（DIG）标记的26个碱基长寡聚核苷酸作为检测探针。结果观察到：在给予电击足底结合噪声的应激刺激之后，尾动脉收缩压逐渐升高，到刺激第5天，刺激组与对照组动物之间尾动脉收缩压差别有统计学显著意义；到刺激第9天，刺激组尾动脉收缩压达最高值；以后各天，血压持续维持在高水平。给予动物电击足底结合噪声的应激刺激4d以后，下丘脑组织中AVP-mRNA水平与对照组水平无显著差异；而给予刺激6d或15d之后，刺激组动物下丘脑组织中AVP-mRNA水平较对照组动物有显著升高（6d：P＜0．005；15d：P＜0．001）。实验中还观察到，实验组与对照组动物之间血浆渗透压无显著差异。上述结果提示：电刺激足底结合噪声的应激刺激在引起大鼠血压升高的同时，还能使下丘脑组织中AVP-mRNA水平升高；AVP-mRNA水平升高的机制有待进一步的研究。
The experiment was performed in SpapeDawlay male rats given chronic stress stimuli. The changes of mRNA of hypothalamic vasopressor (AVP) mRNA in rat hypothalamus were observed by dot blot hybridization and labeled with Digoxigenin (DIG) 26 base-long oligonucleotides as detection probes. The results showed that the systolic pressure of caudal artery increased gradually after the stimulation with foot-binding noise, and the difference of the systolic pressure of caudal artery between the stimulus group and the control group was statistically significant on the fifth day after stimulation. To stimulate the 9th day, stimulate the tail artery systolic pressure up to the highest value; the next day, blood pressure continued to maintain high levels. AVP-mRNA level in hypothalamus tissue was not significantly different from that in control group after stimulated by foot-binding noise for 4 days, while AVP-mRNA level in hypothalamus tissue of stimulation group was significantly higher than that of control group after stimulation for 6 or 15 days The animals in the control group were significantly increased (6d: P <0.005; 15d: P <0.001). Also observed in the experiment, no significant difference in plasma osmolality between the experimental group and the control group of animals. The above results suggest that electrical stimulation of plantar junction noise stimulates AVP-mRNA in hypothalamus while raising the blood pressure in rats. The mechanism of AVP-mRNA increase remains to be further studied .