论文部分内容阅读
本文将过氧化氢酶与经活化处理的醋酸纤维膜共价固定,并与氧电极偶合,研制成静态和流通二种过氧化氢生物电极.研究了醋酸纤维膜的活化、酶的固定化、测试介质等有关的实验条件和参数、测得静态和流通二种电极响应的线性范围分别为6.7×10~(-5)～2.0×10~(-3)mol/L和1.3×10~(-1)～1.0×10~(-3)mol/L.将电极用于实际试样中的回收率测定时,获得了较好的结果.
In this paper, catalase and activated cellulose acetate membrane covalently fixed and coupled with the oxygen electrode, developed into static and circulating two kinds of hydrogen peroxide bioelectrode.Studied the cellulose acetate membrane activation, enzyme immobilization, Test medium and other related experimental conditions and parameters. The linear range of the static and the flow-through electrodes was 6.7 × 10 -5 ~ 2.0 × 10 -3 mol / L and 1.3 × 10 ~ -1) ~ 1.0 × 10 ~ (-3) mol / L. Good results were obtained when the electrode was used to determine the recovery of the sample.