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在胎儿组织中存在一类低分子肿瘤抑制物。胎肝细胞的甲醇－丙酮提取物中保留有大部分抑瘤活性。用体外液体培养条件下对人急性粒系白血病细胞系（ＨＬ－６０）的抑制作用为指标，跟踪指导分离过程。提取物经反相Ｃ１８中压液相色谱、ＳｅｐｈａｄｅｘＬＨ－２０凝胶色谱及氨基键合相高效液相色谱分离得到一纯活性物质，经ＮＭＲ和ＭＳ鉴定为７－酮基胆固醇（７－ｋｅｔｏｃｂｌｅｓｔｅｒｏｌ，７－ＫＣ）。体外琼脂培养条件下７－ＫＣ对小鼠ＷＥＨ１－３和Ｓ－１８０细胞较对正常小鼠骨髓粒一巨噬系祖细胞有更强的抑制增殖及集落生成作用。７－ＫＣ对ＨＬ－６０细胞增殖较对正常人骨髓ＣＦＵ－ＧＭ有更强的抑制作用。
There is a class of low molecular tumor suppressors in fetal tissue. The majority of the antitumor activity remains in the methanol-acetone extract of fetal liver cells. Inhibition of human acute myeloid leukemia cell line (HL-60) under liquid culture in vitro as an indicator, follow-up guide the separation process. The extracts were separated and purified by reversed-phase C18 medium-pressure liquid chromatography, Sephadex LH-20 gel chromatography and amino-bonded phase high performance liquid chromatography. The pure compounds were identified by NMR and MS as 7-ketocblesterol 7-KC). In vitro agar culture conditions 7-KC on mouse WEH1-3 and S-180 cells than normal mouse bone marrow granulocyte macrophage progenitor cells have a stronger inhibitory effect on proliferation and colony formation. 7-KC on HL-60 cell proliferation than normal human bone marrow CFU-GM have a stronger inhibitory effect.