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目的 :研究转人内皮抑素基因双歧杆菌液对血管生长的抑制作用 ,并观察其对动物移植性肝癌Heps的抑瘤作用。 方法 :以小鼠移植性肝癌Heps建立肿瘤模型 ;分别进行灌胃和尾静脉注射给药 ,同时用转人内皮抑素基因双歧杆菌液做鸡胚绒毛尿囊膜 (CAM )实验 ,在解剖显微镜下计数血管条数 ,用图像分析仪定量分析血管密度。实验动物处死后 ,称瘤重并计算抑瘤率。结果 :转基因双歧杆菌液高、中、低 3个剂量组的抑瘤率分别为 49.78%、45 .81%和 30 .41%;普通双歧杆菌组抑瘤率为10 .9%,尾静脉注射转基因双歧杆菌组抑瘤率为 5 8.3%;CAM实验显示转基因双歧杆菌液可明显抑制血管的生成。结论 :转基因双歧杆菌有良好的抑制血管生长和抑制肿瘤生长的作用 ,且其作用有量效关系。
OBJECTIVE: To study the inhibitory effect of Bifidobacterium endostar-transfected human Bifidobacterium on angiogenesis and to observe its anti-tumor effect on Heps of transplanted liver cancer. Methods: Tumor models were established by transplanted hepatocarcinoma Heps mice. The mice were given gavage and tail vein injection respectively. At the same time, the chick embryo chorioallantoic membrane (CAM) The number of blood vessels was counted under a microscope and the blood vessel density was quantitatively analyzed with an image analyzer. After sacrificed, the animals were weighed and the tumor inhibition rate was calculated. Results: The inhibitory rates of Bifidobacterium Bifidobacterium were 49.78%, 45.81% and 30.41% in high, middle and low dose groups, respectively. The inhibition rate of Bifidobacterium group was 10.9% Intravenous injection of Bifidobacterium bifidum group tumor inhibition rate of 5 8.3%; CAM experiments showed that Bifidobacterium Bifidobacterium can significantly inhibit the formation of blood vessels. Conclusion: Bifidobacterium bifidum has a good effect of inhibiting blood vessel growth and inhibiting tumor growth, and its effect has a dose-effect relationship.