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为估计前列环素在冠状血管的分泌率,曾依靠心导管插管来测定其代谢产物。为证实这种检查及冠状动脉造影本身也可能干扰前列环素的内源性生物合成,作者运用质谱分析法,在接受心脏导管插入术的病人中测定流经冠状血管床的血浆6-酮-前列腺素(PG)F_(1α)水平,同时也分析了主要从尿中排泄的代谢产物2,3-dinor-keto-PGF_(1α)(PGI-M)含量。方法:体内实验。共有4组。A 组由8例38～56岁的接受左心导管插入术的男性患者组成。检查包括左心室和冠状血管造影。收集术前2小时、包括插管时在内的2小时及其后2小时的尿液,测定尿中前列环素代谢产物。B 组由4例48～59岁患者组成,他们只接受电生理研究,事前未给予药物。按照与 A 组相同的方式收集尿液,仅第二个2小时延长为4小时。C 组为7名健康男性志愿者,年龄27～34岁,仅接受静脉滴注泛影葡胺,分别收集注射前2
To estimate the secretion of prostacyclin in the coronary vessels, cardiac catheterization has been used to determine its metabolites. To confirm that this test and coronary angiography itself may interfere with the endogenous biosynthesis of prostacyclin, the authors used mass spectrometry to determine plasma 6-keto-cholesterol levels in coronary vascular beds in patients undergoing cardiac catheterization. Prostaglandin (PG) F_ (1α) levels were also analyzed. The content of 2,3-dinor-keto-PGF_ (1α) (PGI-M), which is mainly excreted from the urine, was also analyzed. Methods: In vivo experiments. There are 4 groups. Group A consisted of 8 male patients aged 38-56 who underwent left heart catheterization. Checks include left ventricular and coronary angiography. Urine was collected from urinary prostacyclin metabolites 2 hours prior to surgery, including 2 hours after intubation and 2 hours thereafter. Group B consisted of 4 patients 48 to 59 years of age who underwent electrophysiological studies without medication. Urine was collected in the same manner as in group A, with an extension of only 4 hours for the second 2 hours. Group C, 7 healthy male volunteers, aged 27 to 34 years, received only intravenous infusion of diatrizoate, respectively, before and 2