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自70年代起,国内外已将约氏疟原虫(Plasmodium yoelii)—斯氏按蚊系统用作抗疟药实验研究的动物模型。在实验研究中获得稳定的和较高的蚊媒感染率是十分需要的。约氏疟原虫的孢子增殖受多种因素的影响,作者就小鼠切脾在约氏疟原虫感染过程中对斯氏按蚊感染力变化的影响、对氨基苯甲酸(PABA)对孢子增殖的作用及约氏疟原虫配子母体感染力的周期节律性变化等诸方面进行了实验研究,结果显示: 1.小鼠切脾后,输血接种约氏疟原虫的感染过程出现明显变化。正常小鼠接种后,原虫密度于第8～12天到达最高峰,以后即陡然下降。于第22天全部转阴,但切脾小鼠输血接种后,第6天原虫密度即达较高水平,第8天即出现死亡,至第20天10只鼠死亡7只,且原虫血症水平一直维持在较高水平。 2.正常小鼠输血感染后,以第2～4天对斯氏按蚊的感染力最好,斯氏按蚊的腺感染率可达40％左右,以后即开始下降;但切脾小鼠输血感染后至第10天供蚊叮咬尚获得较高的腺感染率,下降的幅度显著地小于正常小鼠(P<0.001)。 3.以0.5％、0.05％和0.005％浓度的PABA无论在蚊血餐前或血餐后饲蚊,都使蚊胃的卵囊感染强度明显增加,与对照相比,其增长率为152～288％,其中以0.05％浓度的促进作用最为显著。另外上述三种浓度喂供血鼠则只有0.05％浓度有促进作用,蚊
Since the 1970s, Plasmodium yoelii-An. & Quot; An. & Quot; system has been used both at home and abroad as an animal model for the experimental study of antimalarial drugs. It is highly desirable to obtain stable and high rates of mosquito infection in experimental studies. The sporulation of Plasmodium yoelii was influenced by many factors. The authors studied the effects of splenectomy on the infection of An .S. Mosella in the process of Plasmodium yoelii infection, the effects of PABA on sporulation Effects and maternal placental motility of P. yoelii in rhythmicity, etc. The results showed that: 1. The mice infected with transfusion of Plasmodium yoelii showed significant changes in infection process after splenectomy. After inoculation of normal mice, protozoa density peaked on the 8th to 12th days, then decreased sharply. All became negative on the 22nd day. However, the density of protozoa reached a high level on the 6th day after inoculation of the spleen-transplanted mice, the death occurred on the 8th day, and the death of 10 mice on the 20th day was 7, and the parasitemia The level has been maintained at a high level. After transfection of normal mice, the infectivity of An. An. Sinensis was the best at days 2 to 4, and the infection rate of An. Sinensis was about 40% The rate of gonadal infection was significantly higher for the mosquito bites than that of the normal mice (P <0.001). The oocyst infection intensity of mosquito stomach increased significantly with PABA concentrations of 0.5%, 0.05% and 0.005%, both in pre-meal or after-meal meal feeding. Compared with the control, the growth rate was 152 ~ 288%, of which 0.05% concentration of the most significant role in promoting. In addition the above-mentioned three concentrations of feeding blood only 0.05% of the concentration has a promoting effect mosquito