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为了探讨人白细胞介素 18(hIL 18)的表达对鼻咽癌细胞体内致瘤性的影响 ,我们构建了hIL 18的真核表达载体pcD NA3/hIL 18,转染人鼻咽癌细胞株SUNE细胞 ;然后以转染阳性的SUNE细胞和未转染的SUNE细胞接种到用健康人外周血白细胞免疫重建的SCID鼠 (hu PBL SCID鼠 ) ,观察成瘤情况。结果发现 ,所构建的真核表达载体在鼻咽癌细胞中能高效表达hIL 18;ELISA法测得转染阳性细胞培养上清液中hIL 18的含量为 (85± 10 )pg/ml,而未转染的SUNE细胞的培养上清液中hIL 18的含量低于 5pg/ml。将转染阳性的细胞克隆和未转染的SUNE细胞接种hu PBL SCID小鼠后连续观察 5 0d ,发现前者形成的肿瘤其生长速度明显慢于后者 ,其平均瘤重也有显著性差异 (P <0 0 0 1)。说明在体内hIL 18的表达能有效地抑制鼻咽癌细胞的成瘤作用。
In order to investigate the effect of human IL-18 on the tumorigenicity of NPC cells, we constructed the eukaryotic expression vector pcDNA3 / hIL18 of hIL18 and transfected it into human nasopharyngeal carcinoma cell line SUNE Then, the transfected SUNE cells and the untransfected SUNE cells were inoculated into SCID mice (hu PBL SCID mice) immunized with healthy human peripheral blood leukocytes to observe the tumorigenesis. The results showed that the constructed eukaryotic expression vector highly expressed hIL 18 in nasopharyngeal carcinoma cells; the content of hIL 18 in the culture supernatant of transfected cells was (85 ± 10) pg / ml as measured by ELISA The content of hIL 18 in the culture supernatant of untransfected SUNE cells was lower than 5 pg / ml. The positive cell clones and untransfected SUNE cells were inoculated with hu PBL SCID mice continuously for 50 days. The tumors formed in the former showed a significantly slower growth rate than the latter, and the average tumor weight was also significantly different (P <0 0 0 1). This shows that the expression of hIL 18 in vivo can effectively inhibit the tumorigenicity of nasopharyngeal carcinoma cells.