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华支睾吸虫病在本省浦江、杭州市郊,临海县曾有人体感染报告。1994年8月江山市人民医院医治1例病人,在其胆管引流物中发现40余条华支睾吸虫成虫,该患者无外出史。为了解江山市的华支睾吸虫感染情况,我们在该患者居住地须江镇社后村,开展了华支皋吸虫病的调查。材料和方法一,粪检和血检对象社后村的村民,性别不限。1.粪检随机采集大便样品35份,其中少儿10份,青壮年17份,老年人8份。大便样品采用水洗沉淀集卵法,将加水调后粪便滤入杯内,静置30′,倾去上浮液再加水至杯口,静置30′,重复3次后,吸取沉渣,镜检。2.血清学检查随机采集耳垂血132份,其巾少儿25人,青壮年95人,老年12人,利用快速DotELISA法检测华支睾吸虫病人血清。检测该病试剂盒由四川省寄研所提供。
Clonorchis sinensis in the province Pujiang, Hangzhou suburbs, Linhai County had human infection reports. August 1994 Jiangshan People’s Hospital to treat 1 patient, found in the biliary drainage of more than 40 adult Clonorchis sinensis, the patient had no history of going out. To understand Jiangshan City, Clonorchis sinensis infection, we live in the town where the patient Xijiang community after the village, to carry out the survey of Hua cheng gonorrhea. Materials and methods First, the fecal examination and blood test objects She village after the village, gender is not limited. Stool samples stool samples were randomly collected 35, of which 10 children, young and middle-aged 17, 8 elderly. The stool samples were collected by water washing and sedimentation. After the water was added, the stools were filtered and placed in a cup. The stools were allowed to stand for 30 ’. After the liquid was poured, the water was added to the cup and allowed to stand for 30’. After repeated 3 times, sediment and microscopic examination were taken. 2. serological tests Randomly collected 132 parts of the ear lobe blood, towel children 25, 95 young adults, 12 elderly people, the rapid Dot ELISA method for detection of Clonorchis sinensis serum. Detection of the disease kit provided by the Institute of Sichuan Province.