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从枳壳、四季桔和酸橙花药诱导产生植株已经成功。然而,从甜橙花药诱导形成幼株还未见报道。本文叙述从特洛维塔(Trovita)甜橙的花药诱导形成幼株。 采取“Trovita” (Citrus sinensis Osbeck)甜橙的未成熟花蕾,在70％乙醇中浸5秒钟,进行表面消毒。在无菌条件下除去花瓣,从花蕾中取出约处于单核期的花药,立即接种至培养基的表面。以MS为基本培养基,添加50克/升蔗糖及各种浓度的激动
Plants derived from Citrus aurantium, Siji orange and anther anther have been successful. However, the induction of formation of young plants from anthers of orange flowers has not been reported yet. This article describes the induction of young formation from the anther of Trovita sweet orange. Immature buds of “Trovita” (Citrus sinensis Osbeck) sweet orange were dipped in 70% ethanol for 5 seconds for surface disinfection. The petals are removed under aseptic conditions and the anthers at the mononuclear stage are removed from the buds and immediately inoculated onto the surface of the medium. With MS as a basal medium, 50 g / l sucrose and various concentrations of agonist were added