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目的建立回心草药材的指纹图谱。方法采用反相高效液相色谱,色谱条件为:DikmaPlatisilODSC18柱(250mm×4.6mm,5μm);流动相A:0.5%醋酸乙腈,B:0.5%醋酸水溶液;洗脱方法:0～50min,A为20%～55%;50～60min,A为55%～95%,60～85min,A为95%～100%,保持5min;体积流量1.0mL/min,波长360nm,柱温:30℃。运用中药色谱指纹图谱相似度评价系统2004版(国家药典委员会)进行分析。结果构建了回心草药材的指纹图谱。结论该法准确,重现性好,可作为回心草药材的质控方法。
Objective To establish the fingerprint of Huixin herbal materials. Methods The reversed-phase high performance liquid chromatography was used. The chromatographic conditions were as follows: DikmaPlatisilODSC18 column (250mm×4.6mm, 5μm); mobile phase A: 0.5% acetonitrile acetate, B: 0.5% acetic acid aqueous solution; elution method: 0～50min, A 20% to 55%; 50 to 60 minutes, A is 55% to 95%, 60 to 85 minutes, A is 95% to 100% for 5 minutes; volumetric flow rate 1.0mL/minute, wavelength 360nm, column temperature: 30°C. The Chinese version of the chromatographic fingerprint similarity evaluation system version 2004 (National Pharmacopoeia Commission) was used for analysis. Results The fingerprints of Huixin herbal materials were constructed. Conclusion The method is accurate and reproducible, and it can be used as a quality control method for Huixin herbal medicine.